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Submitted on December 11, 2001
Accepted on July 22, 2002
B) BY CORTICOTROPIN RELEASING HORMONE IN MOUSE THYMOCYTES
1 Division of Endocrinology, Children's Hospital, Harvard Medical School, Boston, MA 02115
* To whom correspondence should be addressed. E-mail: karalis{at}a1.tch.harvard.edu.
Corticotropin-Releasing hormone (CRH), a major mediator of the stress response, has been shown to exert potent immunomodulatory effects in vivo, through mechanisms that have not been elucidated yet. To determine the molecular pathways mediating the proinflammatory effects of peripheral CRH, we studied its role in the activation of nuclear factor-B (NF-B), a transcription factor crucial for the regulation of a variety of inflammatory mediator genes. Our studies demonstrate that in mouse thymocytes CRH induces the NF-B DNA-binding activity in a time- and dose-dependent manner, with parallel degradation of its inhibitor protein IB
. The effect of CRH is not inhibited by dexamethasone and is mediated by the PKA and PKC signaling pathways. In vivo, we show that CRH-deficient mice respond to lipopolysaccharide administration by reduced activation of thymus NF-B, despite their significantly elevated proinflammatory cytokine and their low corticosterone levels. These findings suggest a putative molecular pathway mediating the proinflammatory effects of peripheral CRH through induction of the NF-B DNA binding activity.
B •
DNA-binding activity •
CRH antagonist •
-helical •
CRH deficient
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