help button home button Endocrine Society Molecular Endocrinology ENDO 08 Sessions Library
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Molecular Endocrinology, doi:10.1210/me.2003-0299
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow NURSA Molecule Pages Link
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pascual-Le Tallec, L.
Right arrow Articles by Lombès, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pascual-Le Tallec, L.
Right arrow Articles by Lombès, M.
Molecular Endocrinology 17 (12): 2529-2542
Copyright © 2003 by The Endocrine Society

Protein Inhibitor of Activated Signal Transducer and Activator of Transcription 1 Interacts with the N-Terminal Domain of Mineralocorticoid Receptor and Represses Its Transcriptional Activity: Implication of Small Ubiquitin-Related Modifier 1 Modification

Laurent Pascual-Le Tallec, Olivier Kirsh, Marie-Christine Lecomte, Say Viengchareun, Maria-Christina Zennaro, Anne Dejean and Marc Lombès

Institut National de la Santé et de la Recherche Médicale (INSERM) Unité (U) 478 (L.P.-L.T., S.V., M.-C.Z., M.L.) and INSERM U409 (M.-C.L.), Institut Fédératif de Recherche Claude Bernard, Faculté de Médecine Xavier Bichat, 75870 Paris cedex 18, France; and INSERM U579 (O.K., A.D.), Institut Pasteur, 75724 Paris cedex 15, France

Address all correspondence and requests for reprints to: Marc Lombes, M.D., Ph.D., Institut National de la Santé et de la Recherche Médicale, U478, Faculté de Médecine Xavier Bichat, 16 rue Henri Huchard, 75870 Paris cedex 18, France. E-mail: mlombes{at}bichat.inserm.fr.

Molecular mechanisms underlying mineralocorticoid receptor (MR)-mediated gene expression are not fully understood but seem to largely depend upon interactions with specific coregulators. To identify novel human MR (hMR) molecular partners, yeast two-hybrid screenings performed using the N-terminal domain as bait, allowed us to isolate protein inhibitor of activated signal transducer and activator of transcription (PIAS)1 and PIASxß, described as SUMO (small ubiquitin-related modifier) E3-ligases. Specific interaction between PIAS1 and hMR was confirmed by glutathione-S-transferase pull-down experiments and N-terminal subdomains responsible for physical contacts were delineated. Transient transfections demonstrated that PIAS1 is a corepressor of aldosterone-activated MR transactivation but has no significant effect on human glucocorticoid receptor transactivation. The agonist or antagonist nature of the bound ligand also determines PIAS1 corepressive action. We provided evidence that PIAS1 conjugated SUMO-1 to hMR both in vitro and in vivo. Deciphering the unique sumoylation pattern of hMR, which possesses five consensus SUMO-1 binding sites, by combinatorial lysine substitutions, revealed a major impact of sumoylation on hMR properties. Using a murine mammary tumor virus promoter, PIAS1 action was independent of sumoylation whereas with glucocorticoid response element promoter, PIAS1 corepressive action depended on hMR sumoylation status. Taken together, our results identify a novel function for PIAS1 which interacts with the N-terminal domain of hMR and represses its ligand-dependent transcriptional activity, at least in part, through SUMO modifications.

NURSA Molecule Pages Link:

Nuclear Receptors:   MR
Coregulators:   ARIP3  |  PIAS1
Ligands:   Hydrocortisone  |  Aldosterone



This article has been cited by other articles:


Home page
 J Mol EndocrinolHome page
J. B Pippal and P. J Fuller
Structure-function relationships in the mineralocorticoid receptor
J. Mol. Endocrinol., December 1, 2008; 41(6): 405 - 413.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. R. Holmstrom, S. Chupreta, A. Y.-L. So, and J. A. Iniguez-Lluhi
SUMO-Mediated Inhibition of Glucocorticoid Receptor Synergistic Activity Depends on Stable Assembly at the Promoter But Not on DAXX
Mol. Endocrinol., September 1, 2008; 22(9): 2061 - 2075.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. M. Tremblay, B. J. Wilson, X.-J. Yang, and V. Giguere
Phosphorylation-Dependent Sumoylation Regulates Estrogen-Related Receptor-{alpha} and -{gamma} Transcriptional Activity through a Synergy Control Motif
Mol. Endocrinol., March 1, 2008; 22(3): 570 - 584.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. R. Daniel, E. J. Faivre, and C. A. Lange
Phosphorylation-Dependent Antagonism of Sumoylation Derepresses Progesterone Receptor Action in Breast Cancer Cells
Mol. Endocrinol., December 1, 2007; 21(12): 2890 - 2906.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. J. Brayman, N. Dharmaraj, E. Lagow, and D. D. Carson
MUC1 Expression Is Repressed by Protein Inhibitor of Activated Signal Transducer and Activator of Transcription-y
Mol. Endocrinol., November 1, 2007; 21(11): 2725 - 2737.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
A.-N. Takeda, G. M. Pinon, M. Bens, J. Fagart, M.-E. Rafestin-Oblin, and A. Vandewalle
The Synthetic Androgen Methyltrienolone (R1881) Acts as a Potent Antagonist of the Mineralocorticoid Receptor
Mol. Pharmacol., February 1, 2007; 71(2): 473 - 482.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Yokota, H. Shibata, I. Kurihara, S. Kobayashi, N. Suda, A. Murai-Takeda, I. Saito, H. Kitagawa, S. Kato, T. Saruta, et al.
Coactivation of the N-terminal Transactivation of Mineralocorticoid Receptor by Ubc9
J. Biol. Chem., January 19, 2007; 282(3): 1998 - 2010.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
N. Z. Lu, S. E. Wardell, K. L. Burnstein, D. Defranco, P. J. Fuller, V. Giguere, R. B. Hochberg, L. McKay, J.-M. Renoir, N. L. Weigel, et al.
International Union of Pharmacology. LXV. The Pharmacology and Classification of the Nuclear Receptor Superfamily: Glucocorticoid, Mineralocorticoid, Progesterone, and Androgen Receptors
Pharmacol. Rev., December 1, 2006; 58(4): 782 - 797.
[Full Text] [PDF]

Home page
 Genes Dev.Home page
X. Lu, S. Liu, and T. B. Kornberg
The C-terminal tail of the Hedgehog receptor Patched regulates both localization and turnover
Genes & Dev., September 15, 2006; 20(18): 2539 - 2551.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. E. Gomez-Sanchez, A. F. de Rodriguez, D. G. Romero, J. Estess, M. P. Warden, M. T. Gomez-Sanchez, and E. P. Gomez-Sanchez
Development of a Panel of Monoclonal Antibodies against the Mineralocorticoid Receptor
Endocrinology, March 1, 2006; 147(3): 1343 - 1348.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. Huang, X. Li, C. A. Maguire, R. Hilf, R. A. Bambara, and M. Muyan
Binding of Estrogen Receptor {beta} to Estrogen Response Element in Situ Is Independent of Estradiol and Impaired by Its Amino Terminus
Mol. Endocrinol., November 1, 2005; 19(11): 2696 - 2712.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
L. Pascual-Le Tallec and M. Lombes
The Mineralocorticoid Receptor: A Journey Exploring Its Diversity and Specificity of Action
Mol. Endocrinol., September 1, 2005; 19(9): 2211 - 2221.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
L. Pascual-Le Tallec, F. Simone, S. Viengchareun, G. Meduri, M. J. Thirman, and M. Lombes
The Elongation Factor ELL (Eleven-Nineteen Lysine-Rich Leukemia) Is a Selective Coregulator for Steroid Receptor Functions
Mol. Endocrinol., May 1, 2005; 19(5): 1158 - 1169.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
J. Fagart, C. Seguin, G. M. Pinon, and M.-E. Rafestin-Oblin
The Met852 Residue Is a Key Organizer of the Ligand-Binding Cavity of the Human Mineralocorticoid Receptor
Mol. Pharmacol., May 1, 2005; 67(5): 1714 - 1722.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Kim, S. Sharma, Y. Li, E. Cobos, J. J. Palvimo, and S. C. Williams
Repression and Coactivation of CCAAT/Enhancer-binding Protein {epsilon} by Sumoylation and Protein Inhibitor of Activated STATx Proteins
J. Biol. Chem., April 1, 2005; 280(13): 12246 - 12254.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. B. Lee, L. A. Lebedeva, M. Suzawa, S. A. Wadekar, M. Desclozeaux, and H. A. Ingraham
The DEAD-Box Protein DP103 (Ddx20 or Gemin-3) Represses Orphan Nuclear Receptor Activity via SUMO Modification
Mol. Cell. Biol., March 1, 2005; 25(5): 1879 - 1890.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
O. C. Meijer, E. Kalkhoven, S. van der Laan, P. J. Steenbergen, S. H. Houtman, T. F. Dijkmans, D. Pearce, and E. R. de Kloet
Steroid Receptor Coactivator-1 Splice Variants Differentially Affect Corticosteroid Receptor Signaling
Endocrinology, March 1, 2005; 146(3): 1438 - 1448.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. Kurihara, H. Shibata, S. Kobayashi, N. Suda, Y. Ikeda, K. Yokota, A. Murai, I. Saito, W. E. Rainey, and T. Saruta
Ubc9 and Protein Inhibitor of Activated STAT 1 Activate Chicken Ovalbumin Upstream Promoter-Transcription Factor I-mediated Human CYP11B2 Gene Transcription
J. Biol. Chem., February 25, 2005; 280(8): 6721 - 6730.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Perdomo, A. Verger, J. Turner, and M. Crossley
Role for SUMO Modification in Facilitating Transcriptional Repression by BKLF
Mol. Cell. Biol., February 15, 2005; 25(4): 1549 - 1559.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
D. Yamashita, T. Yamaguchi, M. Shimizu, N. Nakata, F. Hirose, and T. Osumi
The transactivating function of peroxisome proliferator-activated receptor {gamma} is negatively regulated by SUMO conjugation in the amino-terminal domain
Genes Cells, November 1, 2004; 9(11): 1017 - 1029.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Marissal-Arvy, M. Lombes, J. Petterson, M.-P. Moisan, and P. Mormede
Gain of Function Mutation in the Mineralocorticoid Receptor of the Brown Norway Rat
J. Biol. Chem., September 17, 2004; 279(38): 39232 - 39239.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
P. Sartorato, F. Cluzeaud, J. Fagart, S. Viengchareun, M. Lombes, and M.-C. Zennaro
New Naturally Occurring Missense Mutations of the Human Mineralocorticoid Receptor Disclose Important Residues Involved in Dynamic Interactions with Deoxyribonucleic Acid, Intracellular Trafficking, and Ligand Binding
Mol. Endocrinol., September 1, 2004; 18(9): 2151 - 2165.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2003 by The Endocrine Society