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A Novel Estrogen Receptor -Associated Protein, Template-Activating Factor Iß, Inhibits Acetylation and Transactivation

Department of Molecular and Integrative Physiology (M.L., A.M.N.), University of Illinois, Urbana, Illinois 61801; and Department of Cell Biology (N.M., J.R.Y.), The Scripps Research Institute, La Jolla, California 92037

Address all correspondence and requests for reprints to: Ann M. Nardulli, Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, 524 Burrill Hall, 407 South Goodwin Avenue, Urbana, Illinois 61801. E-mail: anardull{at}life.uiuc.edu.

Estrogen receptor- (ER) functions as a ligand-activated transcription factor that alters expression of estrogen-responsive genes in target cells. Numerous regulatory proteins interact with ER to influence estrogen-mediated transactivation. We have identified a novel coregulatory protein, template-activating factor-Iß (TAF-Iß), which binds to ER in vitro when the receptor is not complexed with an estrogen response element. The central region of TAF-Iß interacts with both the DNA-binding domain and the carboxy-terminal region of ER. Coimmunoprecipitation experiments demonstrate that TAF-Iß is associated with the unoccupied, but not the estrogen-occupied, ER in MCF-7 breast cancer cells. Overexpression of TAF-Iß inhibits ER-mediated transcription in a dose- dependent manner. TAF-Iß represses p300-mediated acetylation of histones and ER in vitro and decreases ER acetylation in vivo. TAF-Iß also binds to other nuclear receptor superfamily members and represses thyroid hormone receptor ß- induced transcription in transient transfection assays. Taken together, these data provide evidence that TAF-Iß regulates transcription of estrogen- responsive genes by modulating acetylation of histones and ER and that the effects of TAF-Iß extend to other nuclear receptor superfamily members as well.

NURSA Molecule Pages Link:

Nuclear Receptors:   TRβ  |  RXRα  |  ERα  |  PR

Coregulators:   p300

Ligands:   17β-Estradiol  |  9-cis-Retinoic acid  |  Progesterone  |  Thyroid hormone

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