This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wölfl, S. Articles by Majzoub, J. A. Search for Related Content PubMed PubMed Citation Articles by Wölfl, S. Articles by Majzoub, J. A.

Inducible Binding of Cyclic Adenosine 3',5'-Monophosphate (cAMP)-Responsive Element Binding Protein (CREB) to a cAMP-Responsive Promoter in Vivo

Hans-Knöll-Institut für Naturstoff-Forschung (S.W.) 07745 Jena, Germany
Division of Endocrinology (C.M., J.A.M.) Children’s Hospital Harvard Medical School Boston, Massachusetts 02115

In general, DNA-binding factors that activate gene transcription are thought to do so via reversible interaction with DNA. However, most studies, largely performed in vitro, suggest that the transcriptional activator, cAMP response element-binding protein (CREB), is exceptional in that it is constitutively bound to the promoter, where its phosphorylation leads to the recruitment of CREB-binding protein (CBP) to form a CREB/CBP/promoter complex. We have studied how CREB interacts with DNA in vivo to regulate the cAMP-responsive gene encoding human CRH (hCRH). Protein-DNA complexes were cross-linked in cells expressing the endogenous hCRH gene by exposure to a 10 nsec pulse of high-energy UV-laser light, followed by immunoaffinity purification of CREB-DNA complexes. Binding of CREB to a fragment of the hCRH promoter containing a canonical, functional cAMP response element was absent in untreated cells, but was specifically induced after activation of the protein kinase A pathway with forskolin. These data indicate that, in vivo, CREB, like the majority of other DNA-binding transcriptional activators, undergoes signal-mediated promoter interaction.

This article has been cited by other articles:

				Y. Liu, A. Kamitakahara, A. J. Kim, and G. Aguilera Cyclic Adenosine 3',5'-Monophosphate Responsive Element Binding Protein Phosphorylation Is Required But Not Sufficient for Activation of Corticotropin-Releasing Hormone Transcription Endocrinology, July 1, 2008; 149(7): 3512 - 3520. [Abstract] [Full Text] [PDF]

				P. S. Singru, E. Sanchez, R. Acharya, C. Fekete, and R. M. Lechan Mitogen-Activated Protein Kinase Contributes to Lipopolysaccharide-Induced Activation of Corticotropin-Releasing Hormone Synthesizing Neurons in the Hypothalamic Paraventricular Nucleus Endocrinology, May 1, 2008; 149(5): 2283 - 2292. [Abstract] [Full Text] [PDF]

				M. Yao, M. Stenzel-Poore, and R. J. Denver Structural and Functional Conservation of Vertebrate Corticotropin-Releasing Factor Genes: Evidence for a Critical Role for a Conserved Cyclic AMP Response Element Endocrinology, May 1, 2007; 148(5): 2518 - 2531. [Abstract] [Full Text] [PDF]

				T. Sugawara, N. Sakuragi, and H. Minakami CREM confers cAMP responsiveness in human steroidogenic acute regulatory protein expression in NCI-H295R cells rather than SF-1/Ad4BP. J. Endocrinol., October 1, 2006; 191(1): 327 - 337. [Abstract] [Full Text] [PDF]

				B. M. Mayr, E. Guzman, and M. Montminy Glutamine Rich and Basic Region/Leucine Zipper (bZIP) Domains Stabilize cAMP-response Element-binding Protein (CREB) Binding to Chromatin J. Biol. Chem., April 15, 2005; 280(15): 15103 - 15110. [Abstract] [Full Text] [PDF]

				B. F. Clem, E. A. Hudson, and B. J. Clark Cyclic Adenosine 3',5'-Monophosphate (cAMP) Enhances cAMP-Responsive Element Binding (CREB) Protein Phosphorylation and Phospho-CREB Interaction with the Mouse Steroidogenic Acute Regulatory Protein Gene Promoter Endocrinology, March 1, 2005; 146(3): 1348 - 1356. [Abstract] [Full Text] [PDF]

				E Yamamori, M Asai, M Yoshida, K Takano, K Itoi, Y Oiso, and Y Iwasaki Calcium/calmodulin kinase IV pathway is involved in the transcriptional regulation of the corticotropin-releasing hormone gene promoter in neuronal cells J. Mol. Endocrinol., December 1, 2004; 33(3): 639 - 649. [Abstract] [Full Text] [PDF]

				H. Cha-Molstad, D. M. Keller, G. S. Yochum, S. Impey, and R. H. Goodman Cell-type-specific binding of the transcription factor CREB to the cAMP-response element PNAS, September 14, 2004; 101(37): 13572 - 13577. [Abstract] [Full Text] [PDF]

				T. L.-A. Nguyen, C. Calomme, G. Wijmeersch, S. Nizet, E. Veithen, D. Portetelle, Y. de Launoit, A. Burny, and C. Van Lint Deacetylase Inhibitors and the Viral Transactivator TaxBLV Synergistically Activate Bovine Leukemia Virus Gene Expression via a cAMP-responsive Element- and cAMP-responsive Element-binding Protein-dependent Mechanism J. Biol. Chem., August 13, 2004; 279(33): 35025 - 35036. [Abstract] [Full Text] [PDF]

				D. Sawka-Verhelle, L. Escoubet-Lozach, A. L. Fong, K. D. Hester, S. Herzig, P. Lebrun, and C. K. Glass PE-1/METS, an Antiproliferative Ets Repressor Factor, Is Induced by CREB-1/CREM-1 during Macrophage Differentiation J. Biol. Chem., April 23, 2004; 279(17): 17772 - 17784. [Abstract] [Full Text] [PDF]

				A. M. Khan and A. G. Watts Intravenous 2-Deoxy-D-Glucose Injection Rapidly Elevates Levels of the Phosphorylated Forms of p44/42 Mitogen-Activated Protein Kinases (Extracellularly Regulated Kinases 1/2) in Rat Hypothalamic Parvicellular Paraventricular Neurons Endocrinology, January 1, 2004; 145(1): 351 - 359. [Abstract] [Full Text] [PDF]

				Q. Lu, A. E. Hutchins, C. M. Doyle, J. R. Lundblad, and R. P. S. Kwok Acetylation of cAMP-responsive Element-binding Protein (CREB) by CREB-binding Protein Enhances CREB-dependent Transcription J. Biol. Chem., April 25, 2003; 278(18): 15727 - 15734. [Abstract] [Full Text] [PDF]

				M. Nikodemova, J. Kasckow, H. Liu, V. Manganiello, and G. Aguilera Cyclic Adenosine 3',5'-Monophosphate Regulation of Corticotropin-Releasing Hormone Promoter Activity in AtT-20 Cells and in a Transformed Hypothalamic Cell Line Endocrinology, April 1, 2003; 144(4): 1292 - 1300. [Abstract] [Full Text] [PDF]

				L. A. Vereshchagina, M. Tolnay, and G. C. Tsokos Multiple Transcription Factors Regulate the Inducible Expression of the Human Complement Receptor 2 Promoter J. Immunol., May 15, 2001; 166(10): 6156 - 6163. [Abstract] [Full Text] [PDF]

				S. C. Pandey, A. Roy, and N. Mittal Effects of Chronic Ethanol Intake and Its Withdrawal on the Expression and Phosphorylation of the CREB Gene Transcription Factor in Rat Cortex J. Pharmacol. Exp. Ther., March 1, 2001; 296(3): 857 - 868. [Abstract] [Full Text]

				L. F. Michael, H. Asahara, A. I. Shulman, W. L. Kraus, and M. Montminy The Phosphorylation Status of a Cyclic AMP-Responsive Activator Is Modulated via a Chromatin-Dependent Mechanism Mol. Cell. Biol., March 1, 2000; 20(5): 1596 - 1603. [Abstract] [Full Text]